high-throughput bar-coded pyrosequencing Search Results


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Figure 1. Neighbor-joining (NJ) trees based on Kimura-2-Parameter (K2P) distances for <t>cytochrome</t> <t>c</t> <t>oxidase</t> <t>I</t> <t>(COI)</t> mini-barcodes of old museum Lepidoptera specimens. The red markers indicate the position of generated pyrosequences in comparison to the reference library, with blue markers, from BOLD or Genbank. Collection dates are shown in parentheses. doi:10.1371/journal.pone.0021252.g001
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Figure 1. Neighbor-joining (NJ) trees based on Kimura-2-Parameter (K2P) distances for <t>cytochrome</t> <t>c</t> <t>oxidase</t> <t>I</t> <t>(COI)</t> mini-barcodes of old museum Lepidoptera specimens. The red markers indicate the position of generated pyrosequences in comparison to the reference library, with blue markers, from BOLD or Genbank. Collection dates are shown in parentheses. doi:10.1371/journal.pone.0021252.g001
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Figure 1. Neighbor-joining (NJ) trees based on Kimura-2-Parameter (K2P) distances for <t>cytochrome</t> <t>c</t> <t>oxidase</t> <t>I</t> <t>(COI)</t> mini-barcodes of old museum Lepidoptera specimens. The red markers indicate the position of generated pyrosequences in comparison to the reference library, with blue markers, from BOLD or Genbank. Collection dates are shown in parentheses. doi:10.1371/journal.pone.0021252.g001
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Figure 1. Neighbor-joining (NJ) trees based on Kimura-2-Parameter (K2P) distances for <t>cytochrome</t> <t>c</t> <t>oxidase</t> <t>I</t> <t>(COI)</t> mini-barcodes of old museum Lepidoptera specimens. The red markers indicate the position of generated pyrosequences in comparison to the reference library, with blue markers, from BOLD or Genbank. Collection dates are shown in parentheses. doi:10.1371/journal.pone.0021252.g001
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Figure 1. Neighbor-joining (NJ) trees based on Kimura-2-Parameter (K2P) distances for <t>cytochrome</t> <t>c</t> <t>oxidase</t> <t>I</t> <t>(COI)</t> mini-barcodes of old museum Lepidoptera specimens. The red markers indicate the position of generated pyrosequences in comparison to the reference library, with blue markers, from BOLD or Genbank. Collection dates are shown in parentheses. doi:10.1371/journal.pone.0021252.g001
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Ploidy of isolates and spore clones. (A) Representative flow cytometry plots of YJS5845 (left), YJS5885 (right), and derived spore clones. All spore clones of YJS5845 and YJS5885 tested were diploid and haploid, respectively. The black arrows show the position of 1n, 2n, and 4n <t>DNA</t> content. Inset shows percentage of single cells, small-budded cells, and large-budded cells assessed by light microscopy. (B) Whole-genome <t>sequencing</t> was performed for YJS5845 and spore clones (Materials and Methods). YJS5845, and spore clones 5845-18a and 5845-28b, displayed aneuploidy for chromosomes (Chr) XIV, XI, and I, respectively.
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Ploidy of isolates and spore clones. (A) Representative flow cytometry plots of YJS5845 (left), YJS5885 (right), and derived spore clones. All spore clones of YJS5845 and YJS5885 tested were diploid and haploid, respectively. The black arrows show the position of 1n, 2n, and 4n <t>DNA</t> content. Inset shows percentage of single cells, small-budded cells, and large-budded cells assessed by light microscopy. (B) Whole-genome <t>sequencing</t> was performed for YJS5845 and spore clones (Materials and Methods). YJS5845, and spore clones 5845-18a and 5845-28b, displayed aneuploidy for chromosomes (Chr) XIV, XI, and I, respectively.
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Ploidy of isolates and spore clones. (A) Representative flow cytometry plots of YJS5845 (left), YJS5885 (right), and derived spore clones. All spore clones of YJS5845 and YJS5885 tested were diploid and haploid, respectively. The black arrows show the position of 1n, 2n, and 4n <t>DNA</t> content. Inset shows percentage of single cells, small-budded cells, and large-budded cells assessed by light microscopy. (B) Whole-genome <t>sequencing</t> was performed for YJS5845 and spore clones (Materials and Methods). YJS5845, and spore clones 5845-18a and 5845-28b, displayed aneuploidy for chromosomes (Chr) XIV, XI, and I, respectively.
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Ploidy of isolates and spore clones. (A) Representative flow cytometry plots of YJS5845 (left), YJS5885 (right), and derived spore clones. All spore clones of YJS5845 and YJS5885 tested were diploid and haploid, respectively. The black arrows show the position of 1n, 2n, and 4n <t>DNA</t> content. Inset shows percentage of single cells, small-budded cells, and large-budded cells assessed by light microscopy. (B) Whole-genome <t>sequencing</t> was performed for YJS5845 and spore clones (Materials and Methods). YJS5845, and spore clones 5845-18a and 5845-28b, displayed aneuploidy for chromosomes (Chr) XIV, XI, and I, respectively.
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Image Search Results


Figure 1. Neighbor-joining (NJ) trees based on Kimura-2-Parameter (K2P) distances for cytochrome c oxidase I (COI) mini-barcodes of old museum Lepidoptera specimens. The red markers indicate the position of generated pyrosequences in comparison to the reference library, with blue markers, from BOLD or Genbank. Collection dates are shown in parentheses. doi:10.1371/journal.pone.0021252.g001

Journal: PloS one

Article Title: Pyrosequencing for mini-barcoding of fresh and old museum specimens.

doi: 10.1371/journal.pone.0021252

Figure Lengend Snippet: Figure 1. Neighbor-joining (NJ) trees based on Kimura-2-Parameter (K2P) distances for cytochrome c oxidase I (COI) mini-barcodes of old museum Lepidoptera specimens. The red markers indicate the position of generated pyrosequences in comparison to the reference library, with blue markers, from BOLD or Genbank. Collection dates are shown in parentheses. doi:10.1371/journal.pone.0021252.g001

Article Snippet: Supporting Information Table S1 Fresh Lepidoptera specimens used for testing the Pyrosequencing approach for COI mini-barcodes. (DOCX) Table S2 Old museum Lepidoptera specimens used for testing the Pyrosequencing approach for COI mini-barcodes. (DOCX)

Techniques: Generated, Comparison

Ploidy of isolates and spore clones. (A) Representative flow cytometry plots of YJS5845 (left), YJS5885 (right), and derived spore clones. All spore clones of YJS5845 and YJS5885 tested were diploid and haploid, respectively. The black arrows show the position of 1n, 2n, and 4n DNA content. Inset shows percentage of single cells, small-budded cells, and large-budded cells assessed by light microscopy. (B) Whole-genome sequencing was performed for YJS5845 and spore clones (Materials and Methods). YJS5845, and spore clones 5845-18a and 5845-28b, displayed aneuploidy for chromosomes (Chr) XIV, XI, and I, respectively.

Journal: Genetics

Article Title: Incompatibilities in Mismatch Repair Genes MLH1-PMS1 Contribute to a Wide Range of Mutation Rates in Human Isolates of Baker’s Yeast

doi: 10.1534/genetics.118.301550

Figure Lengend Snippet: Ploidy of isolates and spore clones. (A) Representative flow cytometry plots of YJS5845 (left), YJS5885 (right), and derived spore clones. All spore clones of YJS5845 and YJS5885 tested were diploid and haploid, respectively. The black arrows show the position of 1n, 2n, and 4n DNA content. Inset shows percentage of single cells, small-budded cells, and large-budded cells assessed by light microscopy. (B) Whole-genome sequencing was performed for YJS5845 and spore clones (Materials and Methods). YJS5845, and spore clones 5845-18a and 5845-28b, displayed aneuploidy for chromosomes (Chr) XIV, XI, and I, respectively.

Article Snippet: DNA was barcoded using Illumina Nextera XT and high-throughput DNA sequencing was performed on an Illumina NextSeq500 at the Cornell Biotechnology Resource Center, achieving a 50-fold mean coverage.

Techniques: Clone Assay, Flow Cytometry, Derivative Assay, Light Microscopy, Sequencing